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PhysioIndexR - Physiological and Stress Indices for Crop Evaluation

Crop production systems are increasingly challenged by climate variability, resource limitations, and biotic–abiotic stresses. In this context, stress tolerance indices and physiological trait estimators are essential tools to identify stable and superior genotypes, quantify yield stability under stress versus non-stress conditions, and understand plant adaptive responses. The 'PhysioIndexR' package provides a unified framework to compute commonly used stress indices, physiological traits, and derived metrics that are critical in crop improvement, crop physiology, and other agricultural sciences. The package includes functions to calculate classical stress tolerance indices (See Lamba et al., 2023; <doi:10.1038/s41598-023-37634-8>) such as Tolerance (TOL), Stress Tolerance Index (STI), Stress Susceptibility Percentage Index (SSPI), Yield Index (YI), Yield Stability Index (YSI), Relative Stress Index (RSI), Mean Productivity (MP), Geometric Mean Productivity (GMP), Harmonic Mean (HM), Mean Relative Performance (MRP), and Percent Yield Reduction (PYR), along with a convenience wrapper all_indices() that returns all indices simultaneously. The function mfvst_from_indices() integrates these indices into a composite stress score using direction-aware membership values (0–1 scaling) and also averaging, facilitating genotype ranking and selection (See Vinu et al., 2025; <doi:10.1007/s12355-025-01595-1>). The package also implements two novel composite functions: WMFVST(), which computes the Weighted Mean Membership Function Value for Stress Tolerance, and WASI(), which computes the Weighted Average Stress Index, both derived from membership function values (MFV) and raw stress index values, respectively. Beyond stress indices, the package provides functions for key physiological traits relevant to sugarcane and other crops: bmap() computes biomass accumulation and partitioning between leaf, cane/shoot, and root fractions. chl() estimates total chlorophyll content from Soil-Plant Analysis Development (SPAD) and Chlorophyll Content Index (CCI) values using validated quadratic models particularly for sugarcane (See Krishnapriya et al., 2020; <doi:10.37580/JSR.2019.2.9.150-163>). ctd() calculates canopy temperature depression (CTD) from ambient and canopy temperatures, an important indicator of transpiration efficiency. growth() computes key growth analysis parameters, including Leaf Area Index (LAI), Net Assimilation Rate (NAR), and Crop Growth Rate (CGR) across crop growth stages (See Watson, 1958; <doi:10.1093/oxfordjournals.aob.a083596>). ranking() provides flexible ranking utilities for genotype performance with multiple tie-handling and NA-placement options. Through these tools, the package enables researchers to: (i) quantify crop responses to stress environments, (ii) partition physiological components of yield, (iii) integrate multiple indices into composite metrics for genotype evaluation, and (iv) facilitate informed decision making in breeding pipelines, and plant physiology experiments. By combining physiology-based traits with quantitative stress indices, 'PhysioIndexR' supports comprehensive crop evaluation and helps researchers identify multi-stress-resilient superior genotypes, thereby contributing to genetic improvement and ensuring sustainable production of food, fuel, and fibre in the era of limited resources and climate change.

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CANE - Comprehensive Groups of Experiments Analysis for Numerous Environments

In many cases, experiments must be repeated across multiple seasons or locations to ensure applicability of findings. A single experiment conducted in one location and season may yield limited conclusions, as results can vary under different environmental conditions. In agricultural research, treatment × location and treatment × season interactions play a crucial role. Analyzing a series of experiments across diverse conditions allows for more generalized and reliable recommendations. The 'CANE' package facilitates the pooled analysis of experiments conducted over multiple years, seasons, or locations. It is designed to assess treatment interactions with environmental factors (such as location and season) using various experimental designs. The package supports pooled analysis of variance (ANOVA) for the following designs: (1) 'PooledCRD()': completely randomized design; (2) 'PooledRBD()': randomized block design; (3) 'PooledLSD()': Latin square design; (4) 'PooledSPD()': split plot design; and (5) 'PooledStPD()': strip plot design. Each function provides the following outputs: (i) Individual ANOVA tables based on independent analysis for each location or year; (ii) Testing of homogeneity of error variances among distinct locations using Bartlett’s Chi-Square test; (iii) If Bartlett’s test is significant, 'Aitken’s' transformation, defined as the ratio of the response to the square root of the error mean square, is applied to the response variable; otherwise, the data is used as is; (iv) Combined analysis to obtain a pooled ANOVA table; (v) Multiple comparison tests, including Tukey's honestly significant difference (Tukey's HSD) test, Duncan’s multiple range test (DMRT), and the least significant difference (LSD) test, for treatment comparisons. The statistical theory and steps of analysis of these designs are available in Dean et al. (2017)<doi:10.1007/978-3-319-52250-0> and Ruíz et al. (2024)<doi:10.1007/978-3-031-65575-3>. By broadening the scope of experimental conclusions, 'CANE' enables researchers to derive robust, widely applicable recommendations. This package is particularly valuable in agricultural research, where accounting for treatment × location and treatment × season interactions is essential for ensuring the validity of findings across multiple settings.

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1.00 score 187 downloads

NPBBBDAefficiency - A-Efficiency for Nested Partially Balanced Bipartite Block (NPBBB) Designs

Nested Partially Balanced Bipartite Block (NPBBB) designs involve two levels of blocking: (i) The block design (ignoring sub-block classification) serves as a partially balanced bipartite block (PBBB) design, and (ii) The sub-block design (ignoring block classification) also serves as a PBBB design. More details on constructions of the PBBB designs and their characterization properties are available in Vinayaka et al.(2023) <doi:10.1080/03610926.2023.2251623>. This package calculates A-efficiency values for both block and sub-block structures, along with all parameters of a given NPBBB design.

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1.00 score 181 downloads